Figure 1

Electrophoresis of recombinant protein SlGSTD1 and its kinetic properties. (A) SDS-PAGE electrophoresis of recombinant protein SlGSTD1. The region of the target recombinant protein was indicated by a red box. Lanes from left to right represent: Marker, protein marker, Lane 1, pET-26b(+) protein extract, Lane 2, pET-26b(+)/SlGSTd1 protein extract, Lane 3, pET-26b(+)/SlGSTd1 protein extract induced by isopropyl β-D-thiogalactopyranoside (IPTG), Lane 4, purified SlGSTD1. (B) The Km and Vmax values of SlGSTD1 calculated with CDNB as a substrate. Original blots/gels are presented in Supplementary Fig. 1.