Table 2 Primer pairs used in this study.

From: Metabolism and antioxidant activity of SlGSTD1 in Spodoptera litura as a detoxification enzyme to pyrethroids

Primer name

Primer sequence (5′ → 3′)

Primer usage

SlGSTd1F

CATATG GCTTTAGCTCTATACTAC

cDNA full length clone

SlGSTd1R

CTCGAGCAACTCAGTTTTTGCTTTGA

dsSlGSTd1-F1

GGATCCTAATACGACTCACTATAGGGCAGCCCTTAACATCCA

dsRNA clone of SlGSTd1

dsSlGSTd1-R1

CGGTAGCGTCAATCGTAG

dsSlGSTd1-F2

GCAGCCCTTAACATCCA

dsSlGSTd1-R2

GGATCCTAATACGACTCACTATAGGCGGTAGCGTCAATCGTAG

dsGFP-F1

GGATCCTAATACGACTCACTATAGGCACCCTCGTGACCACCCTG

dsRNA clone of GFP

dsGFP-R1

TTGATGCCGTTCTTCTGCTTG

dsGFP-F2

CACCCTCGTGACCACCCTG

dsGFP-R2

GGATCCTAATACGACTCACTATAGGTTGATGCCGTTCTTCTGCTTG

SlGSTd1qF

TAAGTTGACCTTGGCTGACC

qRT-PCR

SlGSTd1qR

TATCCAGGTGCCGATGTCTT

  1. Nucleotides bold were restriction enzyme sites. Nucleotides underlined were T7 RNA polymerase promoter sequence.
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