Figure 5
Rapamycin induces G1 arrest in the H2B WT, but not in the H2B R95A mutant. (A,B) Briefly, overnight cells were sub-cultured for 3 h and samples were taken for asynchronous growth followed by treatment without and with rapamycin (RAP; 200 ng/ml for 30 min). Cells were washed free of rapamycin, incubated in fresh media, and samples were taken at time zero after the treatment, followed by sampling at 30, 60, and 90 min. Samples were processed using FACS analysis and the image treated by the Flowjo software. The results are representative of two independent analyses.
