Figure 5 | Scientific Reports

Figure 5

From: Fibrillin-1 mutant mouse captures defining features of human primary open glaucoma including anomalous aqueous humor TGF beta-2

Figure 5

Increased Tsk mouse retinal apoptosis. (a) Representative images of apoptotic cell labeling (Annexin-V-FITC-positive; green) that was prominent in the GCL of Tsk (n = 5) but not WT (n = 5) mouse retina (aged 3–12 m; scale bar, 20 µm; top row). Annexin-V-positive labeling was also present in other retinal layers such as INL and ONL (bottom row). (b) Annexin-V labeling intensity was higher (p = 0.00005) in the GCL of Tsk (mean ± SD: 23.48 ± 2.73) than WT (7.87 ± 2.73) mouse retina. (c) Representative image showing TUNEL labeling (TUNEL positive, red) was higher in Tsk than WT mouse retina as early as 3 m and this was still present at 12 m of age (n = 3; scale bar: 20 µm). (d) Representative image of GFAP-positive labeling (red) indicating prominent glial activity in the GCL (RGC) layer of Tsk compared with WT mouse retina (scale bar, 20 µm; n = 5–6 per group).

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