Figure 4
From: Predictive blood biomarkers of sheep pregnancy and litter size
Flowchart showing experimental design and sample collection timepoints during discovery phase. (a) Samples were collected at five timepoints during the discovery phase; Day − 7 refers seven days prior to mating ewes and rams, Day 0 refers to the day of mating, Day 35 refers to 35 days after mating, Day 50 refers to 50 days after mating, and Day 70 refers to 70 days after mating. (b) Experimental groups during the discovery phase included control ewes (CNT) which were not pregnant (n = 32) and pregnant ewes (PRG) with different litter sizes (n = 99). The PRG group consisted of pregnant ewes that delivered one lamb (SNG), pregnant ewes that delivered two lambs (TWN), and pregnant ewes that delivered three or more lambs (TRP). All delivered lambs were healthy and viable.
