Table 2 Student’s t-test of four comparison groups from the discovery dataset.

From: Predictive blood biomarkers of sheep pregnancy and litter size

T-test

 

Day − 7

Day 0

Day 35

Day 50

Day 70

CNT vs PRG

NS

NS

NS

Acetic acid, urea, SM (OH) C24:1, lysoPC a C26:0, lysoPC a C26:1, tryptophan, C3 (propionylcarnitine), carnosine, alpha-aminoadipic acid, putrescine, trimethylamine N-oxide, lysoPC a C18:2, hippuric acid, lysoPC a C14:0, l-arginine, lysoPC a C16:1

Urea, glycine, l-arginine, dimethylamine, formate, dimethyl sulfone, choline, acetic acid, 3-hydroxybutyric acid, acetoacetate, l-alanine, sarcosine, isobutyric acid, l-lysine, creatinine, pyruvic acid, d-mannose, l-serine

CNT vs MLP

NS

Kynurenine, l-ornithine

NS

Urea, acetic acid, SM (OH) C24:1, lysoPC a C26:0, l-arginine, C3 (propionylcarnitine), l-carnitine, tryptophan, lysoPC a C26:1, carnosine, putrescine

Urea, l-arginine, choline, glycine, acetic acid, dimethylamine, formate, 3-hydroxybutyric acid, dimethyl sulfone, acetoacetate, isobutyric acid, l-alanine, sarcosine, pyruvic acid, l-lysine, isoleucine

SNG vs TRP

NS

NS

l-Acetylcarnitine

Methionine

NS

TWN vs TRP

NS

NS

NS

Valine, l-lactic acid, Isobutyric acid

NS

  1. Statistical analysis using t-test revealed significant (p-value < 0.05) serum metabolites of each comparison at five timepoints during the discovery phase. NS Not significant, CNT control open ewes, PRG pregnant ewes, SNG pregnant ewes that delivered one lamb, TWN pregnant ewes that delivered two lambs, TRP pregnant ewes that delivered more than two lambs. Day − 7 refers to 7 days prior to initiation of gestation and day 0 is the start of pregnancy.