Table 4 Partial least squares discriminant analysis (PLS-DA) analysis of four comparison groups from the discovery dataset.

From: Predictive blood biomarkers of sheep pregnancy and litter size

PLS-DA VIP

 

Day − 7

Day 0

Day 35

Day 50

Day 70

CNT vs PRG

NS

NS

Putrescine, butyrate, sarcosine, l-ornithine, acetone, total dimethylarginine, ethanol, l-lysine, C3 (propionylcarnitine), taurine, methanol, trimethylamine N-oxide, isobutyric acid, aspartic acid, 3-hydroxyisovaleric acid

Acetic acid, urea, SM (OH) C24:1, lysoPC a C26:0, lysoPC a C26:1, tryptophan, C3 (propionylcarnitine), carnosine, alpha-aminoadipic acid, putrescine, trimethylamine N-oxide, lysoPC a C18:2, hippuric acid, lysoPC a C14:0, L-arginine

Urea, glycine, acetic acid, l-arginine, dimethyl sulfone, 3-hydroxybutyric acid, ethanol, l-lactic acid, l-lysine, sarcosine, dimethylamine, d-glucose, tyrosine, l-alanine, betaine

CNT vs MLP

Tendency

Urea, l-ornithine, l-lysine, acetoacetate, acetic acid, glycine, kynurenine, 3-hydroxybutyric acid, trans-hydroxyProline, total dimethylarginine, SM C16:0, taurine, l-threonine, methanol, butyrate

Acetic acid, l-ornithine, l-lysine, methanol, taurine, trimethylamine N-oxide, acetone, citric acid, sarcosine, ethanol, isobutyric acid, C0 (Carnitine), aspartic acid, butyrate, total dimethylarginine

Acetic acid, urea, l-arginine, tryptophan, carnosine, 3-hydroxybutyric acid, dimethyl sulfone, trimethylamine N-oxide, l-lysine, l-carnitine, lysoPC a C18:2, l-ornithine, hippuric acid, C0 (Carnitine), methanol

Urea, dimethylamine, l-arginine, glycine, dimethyl sulfone, choline, acetic acid, formate, 3-hydroxybutyric acid, l-alanine, isobutyric acid, acetoacetate, isoleucine, l-lysine, pyruvic acid

SNG vs TRP

NS

NS

NS

NS

Tendency

TWN vs TRP

NS

NS

NS

NS

NS

  1. Multivariate statistical analysis of the discovery dataset using PLS-DA revealed top 15 metabolites that significantly (p-value < 0.05) differentiate between the two comparison groups at each timepoint. NS Not significant, CNT control open ewes, PRG pregnant ewes, SNG pregnant ewes that delivered one lamb, TWN pregnant ewes that delivered two lambs, TRP pregnant ewes that delivered more than two lambs. Day − 7 refers to seven days prior to initiation of gestation and day 0 is the start of pregnancy.