Figure 1
Time-course of changes in cellular parameters and picoPPESI negative ion mode mass spectra in tomato trichome stalk cells under salt stress conditions. An image of tip insertion into the stalk cells (a). Changes in the media water potential and cell water potential (Ψw) (b), cell turgor (Ψp) (c), cell osmotic potential (Ψs) (d), cell volume (Vo) (e), cytoplasmic streaming velocity (f), mitochondrion volume per the cell volume (VMito/Vo) (g) under salt stress conditions. PicoPPESI negative ion mode mass spectra obtained from the stalk cells under control and salt stress conditions at 3 h (h and i). Cyan and red plots indicate control and salt stress treatment, respectively. Bar = 200 μm. For Ψw, Ψp, Ψs, and cytoplasmic streaming velocity, data are means ± SE for 3–32 cells from 3 to 6 plants in each treatment (b, c, d, and f). (e) The Vo data are means ± SE for 11–16 cells from 3 to 6 plants in each treatment. (g) The VMito/Vo data is means ± SE for 5–12 cells from 3 to 6 plants in each treatment. Significant difference at the 0.01 and 0.001 probability levels by t-test is indicated with * and **, respectively. The data represent repeated experiments with 8–32 stalk cells in total from 3 to 6 plants in each treatment (h and i). Hex: hexose; HexP: hexose phosphate; CL: cardiolipin; PC: phosphatidylcholine; PE: phosphatidylethanolamine; PI: phosphatidylinositol; PG: phosphatidylglycerol; TAG: triacylglycerol; PS: phosphatidylserine; MGDG: monogalactosyl diacylglycerol. Line graphs were created with Sigmaplot 13.0.
