Figure 4

Statistics and simulation results of the genome-scale metabolic models (GEMs) of S. rapamycinicus NRRL 5491 (wild-type) and its mutant SRMK07. (a) Number of genes, reactions, and metabolites of the GEMs, SrapWT2040 and SrapUV2010, that represent the wild-type and its mutant SRMK07, respectively. (b) Number of essential genes and essential reactions predicted using SrapWT2040 and SrapUV2010. SrapUV2010 was predicted to have eight additional essential genes, and one additional essential reaction in comparison with SrapWT2040. (c) The growth prediction results using SrapWT2040 in comparison with the reported growth data that involved 17 individual carbon sources (Supplementary Table S4) and 19 individual nitrogen sources (Supplementary Table S5). It should be noted that SrapUV2010 also generated the same prediction accuracy as SrapWT2040.