Figure 5
Prediction of intracellular metabolic flux distributions predicted using the genome-scale metabolic models (GEMs) of S. rapamycinicus NRRL 5491 (wild-type) and its mutant SRMK07. Intracellular metabolic flux distributions of the wild-type and the SRMK07 strain were predicted using parsimonious flux balance analysis (pFBA) of the GEMs. Metabolic fluxes predicted to be increased in the SRMK07 strain, compared to the wild-type, are marked with a red arrow. Direct precursors for rapamycin biosynthesis are shown in blue boxes. Dotted arrows indicate multiple reactions. Metabolite abbreviations are: DCDC 4,5-dihydroxycyclohexa-1,5-dienecarboxylic acid, DHCHC 4,5-dihydroxycyclohex-1-enecarboxylic acid.
