Table 2 Deleted core genes in the SRMK07 strain’s genome.
Core gene predicted by ARTSa | Location in the NRRL 5491 genome (bp) | Protein name | Duplication in wild-typeb | Copy number in wild-type | Duplication in SRMK07 | Copy number in SRMK07 |
|---|---|---|---|---|---|---|
TIGR01235 | 11,590,533–11,593,911 | Pyruvate carboxylase | No | 1 | – | – |
TIGR03442 | 1,160,018–1,160,774 | Ergothioneine biosynthesis protein | No | 1 | – | – |
TIGR03438 | 1,160,770–1,161,736 | Ergothioneine-biosynthetic methyltransferase | No | 1 | – | – |
TIGR00173 | 832,039–833,806 | 2-Succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase | No | 1 | – | – |
TIGR01356 | 4,423,896–4,425,273 11,829,102–11,830,458 | 3-Phosphoshikimate 1-carboxyvinyltransferase | Yes | 2 | No | 1 |
TIGR00753 | 2,012,607–2,013,480 10,469,006–10,469,843 11,556,234–11,557,071 | Undecaprenyl-diphosphatase | Yes | 3 | Yes | 2 |
TIGR01900 | 4,510,510–4,511,581 11,545,436–11,546,543 | Succinyl-diaminopimelate desuccinylase | Yes | 2 | No | 1 |
TIGR01311 | 1,818,956–1,820,474 9,256,421–9,257,969 10,360,222–10,361,722 10,637,461–10,638,994 11,557,789–11,559,295 | Glycerol kinase | Yes | 5 | Yes | 4 |
TIGR01312 | 1,144,115–1,145,573 10,338,941–10,340,453 | Xylulokinase | Yes | 2 | No | 1 |
TIGR01751 | 1,592,678–1,594,019 3,325,824–3,327,171 9,491,404–9,492,772 10,704,812–10,706,060 | Crotonyl-CoA carboxylase/reductase | Yes | 4 | Yes | 3 |
TIGR00119 | 4,045,336–4,045,861 11,950,151–11,950,664 | Acetolactate synthase, small subunit | Yes | 2 | No | 1 |
SHMT | 539,567–540,884 4,104,899–4,106,180 4,264,217–4,265,507 6,883,294–6,884,566 | Serine hydroxymethyltransferase | Yes | 4 | Yes | 3 |
Ribosomal_S14 | 5,224,623–5,224,809 10,874,927–10,875,206 | Ribosomal protein S14p/S29e | Yes | 2 | No | 1 |
TIGR01915 | 8,473,740–8,474,514 9,199,776–9,200,484 10,826,539–10,827,214 12,188,951–12,189,509 | NADPH-dependent F420 reductase | Yes | 4 | Yes | 2 |
