Figure 6

Isoform switching between nonsurviving and surviving patients with sepsis. (A) Volcano plot shows differentially expressed isoforms, and the genes with significant p values and dIFs are labeled with gene names. The dashed lines indicate significance values for dIF = 0.1 and FDR = 0.05. (B) Comparison between gene expression levels and dIF. Genes without significant fold changes but with significant dIF are labeled with gene names. (C) Consequences of isoform switching between nonsurvivors and survivors. (D) Alternative splicing events in isoforms relying on the identification of pairs of isoforms involved in the switch. (E) Genome-wide enrichment of specific consequences for each set of opposing consequences (e.g., domain gain vs loss) by analyzing the fraction of events associated with one consequence. There was no statistically significant difference between survivors and nonsurvivors. (F) Analysis of alternative splicing from the perspective of the individual splice type. IF1 isoform fraction for survivors, and IF2 isoform fraction for nonsurvivors.