Figure 7
Animals used as tumor donors for transplantation. (a) Schematic diagram of SKCM model line in zebrafish. Tg (kita:GalTA4,UAS:mCherry)hzm1 zebrafish was crossed with Tg (UAS:eGFP-H-RAS_G12V)io6 line to express oncogenic human HRAS_G12V driven by the melanocyte cell-specific promoter kita. (b) Representative images of kita:Gal4;eGFP-HRAS-G12V whole fish and nodular tail tumor (1 and 2) used in our study (biopsied and disaggregated for posterior allotransplantation).
