Figure 5

miR-302b expression level in the culture supernatant decreased as iPSCs were induced to differentiate to NPCs. The images of cells (a) before inducing differentiation, that is, iPSCs, and (b) on day 15 of the iPSC-derived NPC-induction process. Blue channel represents the nuclei and green is TUJ1. Scale bar is 50 μm. (c) The level of miR-302b in the culture supernatant continually decreased during the induction of iPSCs to NPC differentiation. Error bar =  ± 3SD.