Figure 2
From: cGAS-STING signaling encourages immune cell overcoming of fibroblast barricades in pancreatic cancer
The infiltration of anti-tumor immune cells is enhanced in the cGAS-STING double-expressing PDAC tumors. (a) Immunohistochemical staining for cGAS, STING, CD8a, CD206, and FOXP3 in representative non-D-positive and D-positive cases. (b) The percentage of CD8a expression positive area in cGAS-STING double-positive (D-positive) tissues versus other (non-D-positive) PDAC tumors. (c) The percentage of CD206 expression positive area in cGAS-STING double-positive (D-positive) tissues versus other (non-D-positive) PDAC tumors. (d) The percentage of FOXP3 expression positive area in cGAS-STING double-positive (D-positive) tissues versus other (non-D-positive) PDAC tumors. (e) RT-qPCR analysis of immune cell markers in 17 FFPE PDAC tissues. Heat map showing the fold increase (versus the lowest value of each gene). Scale bar: 100 µm. Each positive area in the immunohistochemical staining was assessed by morphometry from 3 random microscopic fields (10 × objective). ***p < 0.001. ns non-significant. Statistical significance was determined using the 2-tailed t test. This figure was created using GraphPad Prism 9.3.0 (https://www.graphpad.com/scientific-software/prism/).
