Table 4 Primers used in this study.
From: cGAS-STING signaling encourages immune cell overcoming of fibroblast barricades in pancreatic cancer
Genes | Forward (5′ to 3′) | Reverse (5′ to 3′) |
|---|---|---|
18s | CGCTTCCTTACCTGGTTGAT | GAGCGACCAAAGGAACCATA |
IFNB1 | CTTGGATTCCTACAAAGAAGCAGC | TCCTCCTTCTGGAACTGCTGCA |
IL6 | AGACAGCCACTCACCTCTTCAG | TTCTGCCAGTGCCTCTTTGCTG |
CD8a | CTCCCAAAACAAGCCCAAG | AGGGTGAGGACGAAGGTGT |
CD4 | TGACTGCCAACTCTGACACC | GCACTGAGGGGCTACTACCA |
CD3 | ATTTTCGTCCTTGCTGTTGG | GGCTGGTAGAGCTGGTCATT |
CD19 | GTCTTATGGAAACCCGAGCA | ATAGCCCTCCCCTTCCTCTT |
CD11c | CCCCCATTACTACGAGCAGA | GAACAGCATCACACCACCAC |
XCR1 | CAGCTAGAATACGCCCTGCT | ACTTGACCCCCACGAAGAC |
CD80 | TTGTGATATGCTGCCTGACC | GGGCGTACACTTTCCCTTCT |
CD86 | TGGAACCAACACAATGGAGAG | AACACGCTGGGCTTCATC |
CD206 | CGTTTACCAAATGGCTTCGT | GTACCCATCCTTGCCTTTCA |
CD163 | CCAGAAGGAACTTGTAGCCACAG | CAGGCACCAAGCGTTTTGAGCT |
CD56 | CCGTAGAAGCAAAGCCAGAG | TTTGTCTGTGTGGCGTCATT |
NKp46 | CAGTGAAGCTCCTGGTCACA | AAAAGGTAGGTGCCCCAAGT |
CD33 | CCTGCTCGCTCTTTGTCTCT | GTGGTAGGGTGGGTGTCATT |
CD124 | GCGATGTGTGGAGTTGTTTG | GAAGTCATCCCTGCTGCTCT |
FOXP3 | GCTGGAGAAGGAGAAGCTGA | GGATGATGCCACAGATGAAG |
αSMA | CTATGCCTCTGGACGCACAACT | CAGATCCAGACGCATGATGGCA |
FAPα | AGCTTCCTCGTCCAATTCAG | GGTGGATCTCCTGGTCTTTG |
TIMP-1 | CTGGCATCCTGTTGTTGCT | AGGTCGGAATTGCAGAAGG |
Meflin | GACAGCAACGAGCTGACCTT | AGCGGTTGTGGTTGAGTTG |
STING | GATAAACTGCCCCAGCAGAC | TGCCCACAGTAACCTCTTCC |
TNFA | AGCCCATGTTGTAGCAAACC | GCTGGTTATCTCTCAGCTCCA |
IFNA | GAATCTCTCCTTCCTCCTGTCTG | GCTGGAGCCTTCTGGAACT |
IFNB1 (for FFPE) | TTGCTCTCCTGTTGTGCTTC | TCTGACACTGAAAATTGCTGCT |
IL6 (for FFPE) | ATGCAATAACCACCCCTGAC | GCGCAGAATGAGATGAGTTG |
