Figure 4
From: In vitro and in vivo antimalarial activity and chemical profiling of sugarcane leaves
LC-TOF-(negative mode) MS/MS analysis of the DCM (A) and butanol (B) fractions of sugarcane leave extracts. (C) Exemplar tentative identification of p-coumaric acid (4-hydroxycinnamic acid) from the butanol fraction via MS-Dial tool. Comparison of CID MS/MS spectrum of p-coumaric acid (25 V) with that of reference spectrum in the library. (D) Tentative identification of tricin-7-O-eohesperoside from the butanol fraction via a combination of MS-Dial tool and local sugarcane compound database. CID-MS/MS spectrum (40 V) of mass 637.1784 indicating a major fragment ion at m/z 329.0676 through the loss of rhamnose and glucose. Initial loss of rhamnose (− 146) was also observed on the positive CID-MS/MS (15 V) spectrum (Supplementary Fig. S5) in agreement with data in literature38.
