Figure 1

Egg viability assay. (a) Hatch rate dynamics during the egg development. Numbers of hatched eggs in groups the control, T5 and T10 group are presented as percentage of total number of eggs (mean and standard deviation are given). (b) Proportion of four egg development stages. Proportion of eggs from each stage on the day when last newly hatched eggs were observed in the particular group, that is on day 19, 26 and 35 in control, T5 and T10 groups, respectively. (c) Light micrographs illustrating the different stages of egg development. Freshly laid egg, embryonating egg at cell division stage, eye spot stage, hatched egg with an open operculum after the release of miracidium, dead egg (from left to right).