Figure 10 | Scientific Reports

Figure 10

From: Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host’s body temperature

Figure 10

Inhibition of proteolytic activity in F. hepatica egg extracts using class-specific inhibitors. Proteolytic activity of protein extracts from F. hepatica eggs at 0, 5 and 10 days post laying (T0, T5 and T10, respectively) was assayed in the presence of class-specific inhibitors (EDTA, E-64, AEBSF, pepstatin, MG132) and a combination of all five inhibitors (MIX). Inhibitor of threonine peptidases (MG132) was used only at pH 8.5 due to its non-specific activity against cysteine peptidases in acidic environments. Inhibition of activity is expressed as the percentage of remaining proteolytic activity relative to a non-inhibited control (100%). Mean values are listed with standard deviation.

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