Figure 12
From: Design of a multi-epitope vaccine candidate against Brucella melitensis
(A) Adaptation of optimized codons. (B) The MEV (blue) was inserted into the pET28a (+) vector (black) in silico cloning. The codon-optimized sequence of MEV (2418Â bp) generated by the JCat server was inserted between XhoI (158) and BamHI (2582), forming a clone (7753Â bp) using the SnapGene.
