Figure 4 | Scientific Reports

Figure 4

From: Probing Notch1-Dll4 signaling in regulating osteogenic differentiation of human mesenchymal stem cells using single cell nanobiosensor

Figure 4

Dll4 mRNA is a molecular signature of hMSCs differentiation. (A) Representative bright filed and fluorescent images of single hMSCs under control and induction groups after 5 days. Green: Dll4 mRNA; red: ALP activity; blue: nucleus. Control group: cells were cultured and maintained in the basal medium. Induction group: cells were cultured in basal medium and induced by osteogenic induction medium after 2 days of cell seeding. Images are representative of at least three independent experiments. (B,C) Mean fluorescent intensity of ALP activity and Dll4 mRNA expression after 5 days and 10 days of hMSCs osteogenic induction. ALP activity (B) and Dll4 expression (C) comparison in the control and induction groups, respectively. Data represent over 100 cells in each group and are expressed as mean ± s.e.m. (n = 4, ***p < 0.001, **p < 0.01). Scale bar: 50 μm.

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