Figure 8
DDA:TDB and DOTAP:TDB significantly hinder macrophage viability within 2 h of exposure. 2 × 106/ml THP-1-derived macrophages were incubated at 37 °C with liposomes at 20 μg/ml, over 24 h. At various intervals, 50 μl of the co-culture was placed in 500 μl AxV binding buffer and stained with 5 μl of both annexin V (AxV-FITC) and propidium iodide (PI-PE). 10,000 events analysed via flow cytometry. Results shown as the percentage of cells positive for AxV-FITC (Black bars) and PI-PE (grey bars) for n = 2 (mean+/- SEM). *P ≤ 0.05, **P ≤ 0.005, ***P ≤ 0.0005 and ****P ≤ 0.0001 with two-way ANOVA with Tukey’s multiple comparison test. Negative control = macrophages alone.
