Figure 2
From: Intravital 3D visualization and segmentation of murine neural networks at micron resolution
Feature segmentation method allows for micron-scale 3D visualization of the cortex. (a) A sampled b-scan overlaid with the segmented CNS cell bodies; color encodes depth below the cranial window (b–d) Sampled en face images overlaid with the segmented CNS cell bodies at cortical depth (b) 132 µm, (c) 255 µm, and (d) 380 µm. (e) Visualization of a 3D CNS segmentation mask of brain neural networks in a living mouse at micron-level resolution, with a color-encoded depth of more than 10,000 CNS cell bodies, and their surrounding myelinated axons (grey); volume dimension is 0.7 X 0.7 X 0.4 mm (XYZ).
