Figure 1
Domain organization of lmDegU and overall structure of lmDegUDBD. (A) Domain organization and expression constructs of lmDegU. The phosphorylation site of lmDegU (D55 residue) is indicated by a red star. (B) Amino acid sequence of lmDegU. The α-helices of the lmDegUDBD structure are represented by waves above the lmDegU sequence, and the remaining region defined in the lmDegUDBD structure is shown as lines. The phosphorylation site (D55 residue) and dimerization interface residues of lmDegU are colored red and blue, respectively. The three lmDegU residues (R168, N198, and R212), which were mutated to confirm their critical roles in dsDNA binding, are indicated by magenta circles. (C) Overall structure of a lmDegUDBD monomer (chain A). The lmDegUDBD structure is shown as green ribbons. The T213 and V217 residues, which were mutated to confirm the dimerization interface of lmDegUDBD, are shown as blue spheres. The R168, N198, and R212 residues of lmDegU, which were mutated to confirm their critical roles in dsDNA binding, are indicated by magenta spheres.
