Figure 3
From: Simiate and the focal adhesion kinase FAK1 cooperate in the regulation of dendritogenesis
During neuronal development, FAK1 and Simiate display significant variations in their expression levels and their levels of colocalisation in both, nuclei and dendrites. (A–F) At several developmental stages between div 1 and div 22, the volumes of FAK1 and Simiate as well as the volumes of proteins colocalised were measured in nuclei (A–C) and dendrites (D–F) of primary hippocampal neurons by performing 3-dimensional reconstructions. (A, B) The graphs illustrate the expression of nuclear FAK1 and Simiate. (C) Nuclear Simiate and FAK1 show significant changes in their colocalisation during early neuronal development. (D, E) The expression of FAK1 and Simiate in dendrites varies significantly during development. (F) In dendrites, the colocalisation of FAK1 and Simiate rises significantly past div 18. In all graphs, blue lines indicate significance borders. Two mean values are significantly different if they are completely separated by at least one blue line, n equals 8–12 in all experiments. Coloured bars indicate the approximate dendritic stage. Black: protrusions indifferent. Blue: axon growth. Green: onset of dendritogenesis and dendritogenesis. Yellow: slowing of dendritogenesis. Please note that for technical reasons, the total volumes of dendritic proteins were calculated based on densities and known dendrite lengths. For further details, please also see Fig. 4.
