Figure 4

In both, nuclei and dendrites, the proportion of FAK1 and Simiate as well as of their proteins colocalised changes significantly during development. (A–F) At several developmental stages between div 1 and div 22, primary hippocampal neurons were analysed for the proportions of FAK1, Simiate and their proteins colocalised in nuclei (A–C; µm³/µm³) as well as in dendrites (D–F; µm³/µm). The results show significant changes in the proportions of FAK1 and Simiate during development, thus illustrating that levels of FAK1 and Simiate are regulated independently from cellular growth (cp. Fig. 3). For the different units in the measurements of nuclei (per volume: µm³) and dendrites (per length: µm) numbers do not compare directly though (please also see Fig. 2A/B). In all graphs, blue lines indicate significance borders. Two mean values are significantly different if they are completely separated by at least one blue line, n equals 8–12 in all experiments. Coloured bars indicate the approximate dendritic stage. Black: protrusions indifferent. Blue: axon growth. Green: onset of dendritogenesis and dendritogenesis. Yellow: slowing of dendritogenesis.