Figure 6

Alterations in the localisation of endogenous FAK1 and Simiate following expression of recombinant constructs (part 1). The pictures show representative micrographs of div 8 primary hippocampal neurons after over-night expression of GFP constructs (cp. Fig. 5). For each condition, a neuron and magnifications of parts from a dendrite, from the soma and from the nucleus are shown. The respective areas are indicated by white boxes in the overview-picture on the left. Below each magnification, graphs illustrate the signal intensities as exemplary quantified with ImageJ software for each protein of interest. The results are derived from 2 to 4 experiments. Dotted lines indicate the line of measurement. Scale bars: 1 µm in the magnifications and 2 µm in the overview picture. All images are contrast enhanced to demonstrate the colocalisation of FAK1 and Simiate (yellow). (A,B) Expressed GFP shows no colocalisation with FAK1 or Simiate in dendrites, somata or nuclei. (C) GFP-Simiate attracts some endogenous FAK1 in dendrites, but not in the soma. A strong attraction is seen in the nucleus. Please also see Fig. 7 for further details on the other contructs.