Figure 8
From: Simiate and the focal adhesion kinase FAK1 cooperate in the regulation of dendritogenesis
FAK1 and Simiate function together in dendritogenesis. (A) Dendritic trees experience different degrees of complexity after over-night expression of recombinant FAK1 and Simiate. Neurons expressing the indicated constructs were processed in Fiji software for Sholl analyses based on MAP2 staining and GFP signal. (B–E) Results from Sholl analyses are shown as percentage difference to GFP expression (control). Significant changes to control conditions are indicated by big rhombs, whereas significant differences between constructs are displayed by grey boxes. Small rhombs indicate no significant difference from control, as for instance seen with Simiate constructs (yellow and red lines) beyond 120 µm or in the co-expression experiments (green lines) around 50 µm. Scale bar: 10 µm. GFP: n = 20; FAK1: n = 21; Simiate: n = 21; NLS-Simiate: n = 21; FAK1 + NLS-Simiate: n = 18. For more statistical details, please see Suppl. Table 1.
