Figure 2
From: Efficient transdermal delivery of functional protein cargoes by a hydrophobic peptide MTD 1067
Purification of peptide and recombinant proteins. (a) RP-HPLC analysis of purified GHRP-6 and MTD-GHRP-6 peptides using the 20–60% solvent B gradient method for 25 min. (b) SDS-PAGE and western blot analysis of des(1-3)IGF-I and MTD-des(1-3)IGF-I proteins, (c) SDS-PAGE and western blot analysis of PDGF-BB and MTD-PDGF-BB proteins. M, molecular weight marker; R, reduced form; NR, non-reduced form; *, blank lane without sample loading. The raw data of western blot analysis in (b) and (c), respectively, were presented in Supplementary Information as Fig. S4a,b.
