Figure 3
From: Efficient transdermal delivery of functional protein cargoes by a hydrophobic peptide MTD 1067
Biological activity of the MTD 1067-conjugated peptide. (a) Melanogenesis inhibition activity of GHRP-6 and MTD-GHRP-6 using murine melanoma cell line B16F10. α-MSH, α-melanocyte stimulating hormone. The cell culture treated with α-MSH only in the absence of GHRP-6 and MTD-GHRP-6 was used as the control to calculate the melanogenesis activity (%). Asterisks indicate statistical significance determined by Student's t-test. Anti-melanogenesis activity was confirmed by statistical significance compared the α-MSH-treated cells without the GHRP-6 peptides. (b) Collagen synthesis effect of GHRP-6 and MTD-GHRP-6. The amount of collagen in the HDFa culture supernatant was measured using a procollagen type I C-peptide (PIP) EIA kit. The collagen synthesis effect was confirmed with statistical significance by comparison with the cells cultivated without GHRP-6 and MTD-GHRP-6. Asterisks indicate statistical significance determined by Student's t-test. (*p < 0.05, **p < 0.01, ***p < 0.001).
