Figure 5
From: Efficient transdermal delivery of functional protein cargoes by a hydrophobic peptide MTD 1067
Biological activity of PDGF-BB and MTD-PDGF-BB. (a) Cell proliferation activity of PDGF-BB and MTD-PDGF-BB using fibroblast cell line NIH 3T3 cell. Cell proliferation activity in NIH 3T3 cells was analyzed by measuring the color development using MTT. All data are expressed as mean ± standard deviation based on triplicates of at least two independent experiments. (b) Collagen synthesis effect of PDGF-BB and MTD-PDGF-BB. The amount of collagen in the HDFa culture supernatant was measured using a procollagen type I C-peptide (PIP) EIA kit. Data are expressed as mean ± S.E. based on triplicates of at least two independent experiments. Asterisks indicate statistical significance compared with the cell culture without treatment of PDGF-BB and MTD-PDGF-BB, as determined by the student's t-test (*p < 0.05, **p < 0.01, ***p < 0.001).
