Figure 7

Effects of Wolbachia and B-vitamin supplementation on 45-day survivorship of female C. lectularius used in the fecundity assay. The Wb⁺Vit^– line is obscured by the Wb⁺Vit⁺ line, both at 100%. Wb⁺Vit⁺ and Wb^–Vit⁺ females experienced 50% and 17.3% mortality, respectively (Product-limit survival analysis: Wilcoxon X² = 25.6657, df = 3 p < 0.0001) (A). The average survival age within the 45-day experiment shows that all females in the Wb⁺Vit^– and Wb^–Vit⁺ groups survived for 45 days but survival was significantly lower for the Wb⁺Vit⁺ (One-way ANOVA, Tukey’s honestly significant difference test, F_3,81 = 7.4441, p = 0.0002) (B).