Figure 7

Differentially expressed genes in D55 and D105 maternal blood leukocyte transcriptomes. Blood was collected from the same females on D55 and D105 of gestation. The selection of the dams for this study was based on the group, weight, sex, and number of fetuses at D105 collection. Shown are the genes identified by both EdgeR and DESeq2 as statistically different (p < 0.05) in the named comparisons. AI artificial insemination (i.e., control), IVP-N embryos were produced by in vitro procedures that were < 97% of the control’s weight at D105. IVP-LOS embryos were produced by in vitro procedures and were ≥ 97% of the control’s weight at D105. “604 & 664” are the numbers of the heifers carrying the two largest LOS fetuses. Gene names starting with “LOC” are uncharacterized transcripts. The “No gene-both” designation notes that genes were identified as differentially expressed by either EdgeR or DESeq2 but not both.