Figure 1

Transcript knockdown efficacy overtime of RNAi-based microinjection targeting the white gene of the SGSB. (A) SGSB adults had been treated at < 24 h after emergence. Significant white knockdown \(({F}_{\left(\mathrm{2,69}\right)}=63.64;p<0.0001)\) was observed in adults treated with white dsRNA relative to GFP dsRNA- and buffer-treated controls at 4, 10 and 30 days after treatment (DAT). (B) SGSB adults had been treated during fifth nymphal stage at either less than three or more than 4 days prior to adult emergence (DPE). Significant white knockdown \(({F}_{\left(\mathrm{3,92}\right)}=17.61;p<0.0001)\) was observed at 2, 10 and 30 days after adult emergence (DAE) in SGSB adults treated with white dsRNA either at < 3 DPE or > 4 DPE relative to GFP dsRNA- and buffer-treated controls. In each graph, bars discriminate white normalized relative expression means ± SE per time point and above bars show overall means ± SE per treatment across time. Treatment means followed by the same letter were not statistically different (Fisher’s LSD test, α = 0.05).