Figure 3
Comparison between z-stacked Gaussian beam scanned image and Bessel beam scanned image of a ScaleA2 cleared thy1-YFP-H mouse brain slice. (a) Relative configurations of the objective lens and the fixed slice. (b) 3D reconstructed image from Maximum intensity projection of a 250 µm thick z-stack image of neurons (color-coded by depth) in the sensory cortex measured by Gaussian beam scanning. The neurons were serially numbered in accordance with the z position started from the surface of the brain slice. (c) A xy image of the same field of view in (b) at the focus, 60 µm apart from the slice surface, was collected by scanning a Bessel beam using the light-needle creating device. The same neurons were selected and numbered as in (b). The scale bar along an image was shown as their pixels.
