Table 2 Data of CelC307 purification using Ni–NTA chromatography and single-step thermal shock.

From: Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01

Steps

Volume (ml)

Total protein (mg)

Total activity (U)

Specific activity (U/mg)

Purification (fold)

Yield (%)

Crude extract

4

6.4

82

12.8

1

100

Thermal shock

4

1.4

55

34.3

2.6

67

Ni–NTA

4

2.4

32.7

13.6

1.06

39

Back to article page