Table 3 Comparison of activity, temperature, pH and kinetic parameters of CelC307 with other studied members of glycoside hydrolase family.

From: Expression, characterization, and activity optimization of a novel cellulase from the thermophilic bacteria Cohnella sp. A01

Source

Type/family

Substrate

Temp

pH

Km (mM)

Vmax (U/mg)

kcat (S−1)

kcat/Km (M−1 S−1)

Ref.

CelC307

Endoglucanase

CMC

40 °C

7

0.46

62.58

104.30

226.74

This study

CH43

EG family 5

CMC

65 °C

5

1.5

0.93

–

–

9

HR68

EG family 5

CMC

70 °C

6.5

1.7

1.70

–

–

9

Bacillus subtilis CBS31

Endoglucanase

CMC

50 °C

7.5

0.0183

1293.33

484,998

–

10

Caldibacillus cellulovorans

Cellulase

CMC

80 °C

6.5

3.4

44.7

–

–

11

Bacillus vallismortis RG-07

Cellulase

CMC

65 °C

7

1.923

769.230

–

–

12

Bacillus subtilis BC1

Cellulase

CMC

60 °C

8

1.243

271.3

–

–

13

Penicillium simplicissimum H-11

β-Glucosidase

CMC

60 °C

5

14.881

0.364

–

–

14

Actinomyces sp. KNG

Endo-β-1,4-glucanase

CMC

55 °C

7

0.39%

143

–

–

15

Colletotrichum orchidophilum

Endo-β-1,4-glucanase

CMC

55 °C

5

2.65

290.70

–

75.67

16

Botrytis ricini URM 5627

Endo-β-1,4-glucanase

CMC

50 °C

5

0.1299

0.097

–

–

17

Aspergillus fumigatus GH7

Endo-1,4-β-glucanase

CMC

55 ◦C

6

24.5

6193

5037

205.9

18

Chaetomium thermophilum

Endoglucanase7

CMC

55 °C

5

79.2

59.6

2.11

0.0267

19

Myceliophthora thermophile

    

24.0

622.5

–

0.313667

20

Talaromyces emersonii CBS394.64

Endo-1,3-,4-β-glucanase 7

CMC

70 °C

4.5

20.8

2257

–

78.9

21

Aspergillus fumigatu

Endo-1,3-1,4-β-glucanase 7

CMC

40 °C

4.5

209.7

51.9

43.3

0.2

22

Daldinia eschscholzii

Endoglucanase

CMC

70 °C

6

1.74

0.63

–

0.28

23

Fusarium oxysporum

Endo-1,4-β-d-glucanase

CMC

50 °C

6

23.2

22.5

–

0.55

24

Melanocarpus sp.MTCC 3922

Endoglucanases (EG I and EG II

CMC

50 °C

6

20.0

954

–

31.8

25

Fomitopsis pinicola

Endo-beta-1,4-glucanase family 5

CMC

50 °C

5

12.0

1250

–

55.4

26

Penicillium purpurogenum

Endo-1,4-glucanase

CMC

70 °C

5

1.15

220

–

118

27

Gloeophyllum trabeum CBS 900.73

Glycosyl hydrolase (GH) family 5

CMC

70 °C

4

4.5

1475

–

878

28

Bacillus tequelensis BD69

β-glucosidase

CMC

50 °C

5

7.43

1462

454.05

61.12

29

Chaetomium thermophilum CTendo45

Endoglucanase

CMC

60 ◦C

4

5.93

4.42

0.37

62.06

30

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