Figure 3 | Scientific Reports

Figure 3

From: The cell cycle stage of bovine zygotes electroporated with CRISPR/Cas9-RNP affects frequency of Loss-of-heterozygosity editing events

Figure 3

Representative graphical outline of embryos identified to represent homozygous full edits. Proportion of SNP-heterosygosity along chromosomes 29 is reported for DNA-windows with a red arrow indicating the Cas9 target site. Each spot represents state of heterosygosity of one window encompassing a total of 20 SNP-marker. All in all 11 out of 23 embryos showed no Loss-of-heterozygosity in chromosome 29, containing the target side, and were therefore classified as non-disrupted as demonstrated exemplarily in (A). Conversely, 1 embryo showed Loss-of-heterozygosity along the entire target-side arm of the chromosome and were therefore classified as Loss-of-chromosome (B) whereas 3 embryos displaying losses of heterozygosity distal the target site were classified as Loss-of-chromosomal arms (C). A total of 6 Embryos displaying Loss-of-heterozygosity along large segments within the chromosome were classified as Loss-of-chromosomal section (D).

Back to article page