Figure 6
From: Enhanced propagation of Granulicatella adiacens from human oral microbiota by hyaluronan
Isolation of HA-utilizing bacteria from human oral microbiota. (a) HA degradation by G. adiacens. Cell suspension or supernatant was spotted on the center of the halo-forming plate for 1 day (left). Acetic acid was spread onto the plate for halo formation (right). (b) Assimilation assay of HA by G. adiacens. Growth of G. adiacens in nutrient-poor medium (1/20 × GAM; blue) or the same liquid medium but containing glucose (green) or HA (red) as a carbon source was compared (top). *p < 0.05, significant growth promotion compared to growth in nutrient-poor medium (1/20 × GAM; t test). HA concentrations during growth in HA-containing medium were determined (bottom). The isolated G. adiacens extracellularly secreted HA-degrading enzymes and assimilated HA.
