Figure 3 | Scientific Reports

Figure 3

From: Utilizing novel TBI-on-a-chip device to link physical impacts to neurodegeneration and decipher primary and secondary injury mechanisms

Figure 3

Quantifying the elevation of acrolein-Lys adducts as a function of single-impact severity. Immunocytochemistry (ICC) and immunofluorescence were used to detect and quantify acrolein-Lys adducts in histiotypical neuronal networks at 24 h post-impact, and compared with non-impacted, procedurally and age matched control-networks. (A) Average intensity measurements for Acrolein-Lys adducts given as percent control values for single impacts of 0 (control), 30, 100, and 200 g, (B) with the corresponding averaged acrolein values plotted as a function of acceleration to better illustrate this relationship (R2 = 0.95, p < 0.05). Staining intensity is shown as % control values ± SD (n = 18). One-way ANOVA and subsequent Scheffe post hoc analysis were used to determine significance: * p < 0.01.

Back to article page