Figure 1
From: Mutations in the miR-142 gene are not common in myeloproliferative neoplasms
Characteristics of the MIR142 mutations detected in this study. (A) Locations of the mutations on the secondary stem-loop structure (predicted with mfold37) of the wild-type miR-142 precursor. Blue font indicates miRNA duplex, dark blue indicates seed regions, and black indicates flanking regions and terminal loop. Gray symbols represent mutations identified before (based on4), and red-circled symbols indicate mutations identified in this study. (B) Secondary (above) and 3D (below) RNA structures adopted by the n.33T>C and n.59T>C mutant found in CLL. The 3D mutant structure (blue) is aligned with the corresponding wild-type structure (black). The mutations are indicated in red. The 3D structures were generated using RNAComposer38 and visualized in PyMOL (Schrödinger, LLC, New York, NY, USA). (C) Sanger sequencing results for wild-type and mutated samples.
