Figure 4 | Scientific Reports

Figure 4

From: Development of a UPLC-MRM-based targeted proteomic method to profile subcellular organelle marker proteins from human liver tissues

Figure 4

Subcellular organelle marker profiles characterized by UPLC-MRM. 20 µg total protein were digested for each sample. Relative quantification was achieved using response ratio, which corresponds to the ratio of light peptide area to spiked-in heavy labelled peptide area. Each fraction’s response ratio was normalized to its corresponding PNS response ratio. Solid circles, open circles, and squares represent the average normalized response ratio from three human liver donors, and error bars stand for the standard deviation. Wedge indicates increasing sucrose % from fraction 1 to 12.

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