Figure 10
Fluorescence quenching of HXYN2 by interaction with FA. (A) Stern–Volmer plot of the interaction between HXYN2 and FA at pH 4.0, 7.0, and 9.0. Relative increases of fluorescence intensities at 335 nm (F/F0) versus FA concentration were used to estimate Stern–Volmer constants (Ksv). (B) Plots of Log (F0 − F/F) versus Log (FA) for FA quenching effect on HXYN2 emission fluorescence at pH 4.0, 7.0, and 9.0. (C) Stern–Volmer constants (Ksv), Bimolecular quenching constant (Kq), Binding constants (Kb), and binding sites (n) of HXYN2 in complex with FA at different pHs.
