Figure 1

Synthesis of C-DIM12 and effect of C-DIM12 on motor functions of mice after ICH. (a) The chemical reaction used for the synthesis of C-DIM12. (b–d) Results of performance in the beam-walking test which was evaluated by foot fault rate (b), walking distance (c) and performance score (d). C-DIM12 (50 or 100 mg/kg) or vehicle was orally administered at 3 h, 27 h and 51 h after ICH induction. Concerning the fault rate, significant differences between two groups were observed (two-way repeated measure ANOVA: interactions, F_12,92 = 17.11, P < 0.001; time, F_4,92 = 164.4, P < 0.001; treatment, F_3,23 = 62.96, P < 0.001). Concerning the walking distance, significant differences between two groups were observed (two-way repeated measure ANOVA: interactions, F_12,92 = 9.581, P < 0.001; time, F_4,92 = 87.77, P < 0.001; treatment, F_3,23 = 23.17, P < 0.001). Concerning the performance score, significant differences between two groups were observed (two-way repeated measure ANOVA: interactions, F_12,92 = 11.48, P < 0.001; time, F_4,92 = 125.5, P < 0.001; treatment, F_3,23 = 32.26, P < 0.001). (e) Results of performance in the modified limb-placing test. Significant differences between two groups were observed (two-way repeated measure ANOVA: interactions, F_12,92 = 14.92, P < 0.001; time, F_4,92 = 180.5, P < 0.001; treatment, F_3,23 = 49.86, P < 0.001). Number of mice examined in (b-e) was 4 in sham group, 9 in ICH + vehicle group, 8 in ICH + C-DIM12 (50 mg/kg) group and 6 in ICH + C-DIM12 (100 mg/kg) group, respectively. **P < 0.01, ***P < 0.001 versus sham group, #P < 0.05, ##P < 0.01, ###P < 0.001 versus ICH + vehicle group.