Figure 4
Effects of C-DIM12 and amodiaquine on axonal integrity after ICH. C-DIM12 (50 mg/kg, p.o.), amodiaquine (AQ; 40 mg/kg, i.p.) or vehicle (p.o. or i.p.) was administered at 3, 27 and 51 h after ICH induction. (a) NF-H-immunopositive axons in the internal capsule (IC) at 24 h after ICH. Scale bars = 20 μm. (b) Quantitative results of axon fragmentation at 24 h. Number of mice was 4 in sham group, 6 in ICH + vehicle group, 7 in ICH + C-DIM12 group and 7 in ICH + AQ group, respectively. (c) NF-H-immunopositive axons at 72 h after ICH. Scale bars = 20 μm. (d) Quantitative results of axon fragmentation at 72 h. Number of mice was 3 in sham group, 5 in ICH + vehicle group, 5 in ICH + C-DIM12 group and 4 in ICH + AQ group, respectively. (e) APP immunohistochemistry at 24 h after ICH. Scale bars = 500 μm. H; hematoma. (f) Quantitative results of APP-positive area at 24 h. Number of mice was 4 in sham group, 6 in ICH + vehicle group, 7 in ICH + C-DIM12 group and 7 in ICH + AQ group, respectively. (g) APP immunohistochemistry at 72 h after ICH. Scale bars = 500 μm. H; hematoma (h) Quantitative results of APP-positive area at 72 h. Number of mice was 4 in sham group, 5 in ICH + vehicle group, 5 in ICH + C-DIM12 group and 4 in ICH + AQ group, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham group, #P < 0.05, ##P < 0.01, ###P < 0.001 (ANOVA results: for b, F3,20 = 28.79, P < 0.001; for d, F3,13 = 22.36, P < 0.001; for f, F3,20 = 11.74, P < 0.001; for h, F3,14 = 56.61, P < 0.001).
