Figure 5
Effects of C-DIM12 and amodiaquine on iNOS expression and NO-related oxidative stress after ICH. C-DIM12 (50 mg/kg, p.o.), amodiaquine (AQ; 40 mg/kg, i.p.) or vehicle (p.o. or i.p.) was administered at 3, 27 and 51 h after ICH induction. (a) Expression level of iNOS mRNA was quantified at 6 h after ICH. Number of mice examined was 4 in sham group, 5 in ICH + vehicle group, 5 in ICH + C-DIM12 group and 4 in ICH + AQ group, respectively. (b) Representative images of nitrotyrosine immunohistochemistry at 72 h after ICH. Scale bars = 1 mm. (c) Quantitative results of nitrotyrosine-positive area in the ipsilateral hemisphere. Number of mice examined was 4 in sham group, 7 in ICH + vehicle group (for C-DIM12), 8 in ICH + C-DIM12 group, 5 in ICH + vehicle group (for AQ) and 5 in ICH + AQ group, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham group, #P < 0.05, ##P < 0.01 (ANOVA results: for left panel of a, F2,11 = 30.71, P < 0.001; for right panel of a, F2,10 = 26.64, P < 0.001; for left panel of c, F2,16 = 15.00, P < 0.001; for right panel of c, F2,11 = 41.10, P < 0.001).
