Figure 6
Effect of iNOS inhibitor on NO-related oxidative stress and motor performance after ICH. 1400 W (20 mg/kg) or vehicle was intraperitoneally administered twice daily for six times, from 3 h after ICH induction. (a) Representative images of nitrotyrosine immunohistochemistry. Scale bars = 1 mm. (b) Quantitative results of nitrotyrosine-positive area in the ipsilateral hemisphere. Number of mice examined was 4 in sham group, 6 in ICH + saline group and 7 in ICH + 1400 W group, respectively. ***P < 0.001 versus sham group, #P < 0.05. (ANOVA results: F2,14 = 15.99, P < 0.001) (c-e) Results of performance in the beam-walking test evaluated by foot fault rate (c), walking distance (d) and performance score (e). Results of two-way repeated measure ANOVA were as follows. Concerning the fault rate: interactions, F8,56 = 11.00, P < 0.001; time, F4,56 = 46,80, P < 0.001; treatment, F2,14 = 34.47, P < 0.001. Concerning the walking distance: interactions, F8,56 = 6.830, P < 0.001; time, F4,56 = 28.95, P < 0.001; treatment, F2,14 = 18.18, P < 0.001.. Concerning the performance score: interactions, F8,56 = 11.73, P < 0.001; time, F4,56 = 51.50, P < 0.001; treatment, F2,14 = 35.62, P < 0.001. (f) Results of performance in the modified limb-placing test. Two-way repeated measure ANOVA: interactions, F8,56 = 12.66, P < 0.001; time, F4,56 = 69.43, P < 0.001; treatment, F2,14 = 63.77, P < 0.001. Number of mice examined in (c-f) was 4 in sham group, 6 in ICH + vehicle group and 7 in ICH + 1400 W group, respectively. *P < 0.05, **P < 0.01, ***P < 0.001 versus sham group, #P < 0.05, ##P < 0.01 versus ICH + vehicle group.
