Figure 7
Effect of iNOS inhibitor on ICH-induced neural death, activation of microglia/macrophages and accumulation of astrocytes. 1400 W (20 mg/kg) or vehicle was intraperitoneally administered twice daily for six times, from 3 h after ICH induction. Immunohistochemical examinations for NeuN, Iba1 and GFAP were conducted on coronal brain sections obtained at 72 h after ICH. (a) Representative images of NeuN immunohistochemistry. Magnified views of the regions for quantification obtained from a mouse of sham group, ICH + vehicle group and ICH + 1400 W group are shown. Scale bars = 100 μm. (b) Quantitative results of NeuN-positive cells in the central region of hematoma. (c) Representative images of Iba1 immunohistochemistry. Scale bars = 100 μm. (d) Quantitative results of Iba1-positive cells in the peri-hematoma region. (e) Representative images of GFAP immunohistochemistry. Scale bars = 100 μm. (f) Quantitative results of GFAP-positive area in the peri-hematoma region. Number of mice examined was 4 in sham group, 6 in ICH + vehicle group and 7 in ICH + 1400 W group, respectively. ***P < 0.001 versus sham group, #P < 0.05, ##P < 0.01, ###P < 0.001 (ANOVA results: for b, F2,14 = 172.3, P < 0.001; for d, F2,14 = 63.29, P < 0.001; for f, F2,14 = 57.42, P < 0.001).
