Figure 8
Effect of iNOS inhibitor on axonal integrity after ICH. 1400 W (20 mg/kg) or vehicle was intraperitoneally administered twice daily for six times from 3 h after ICH induction, and immunohistochemical examinations for NF-H and APP were conducted on coronal brain sections obtained at 72 h after ICH. (a) Representative images of NF-H-immunopositive axons in the internal capsule within the hematoma. Scale bars = 20 μm. (b) Quantitative results of the morphology of axonal fibers as axonal shape index. (c) Representative images of APP immunohistochemistry. Scale bars = 500 μm. H; hematoma. (d) Quantitative results of APP-positive area. Number of mice examined was 4 in sham group, 6 in ICH + vehicle group and 7 in ICH + 1400 W group, respectively. *P < 0.05, **P < 0.01 versus sham group, #P < 0.05, n.s., not significant (ANOVA results: for b, F2,14 = 85.88, P < 0.001; for d, F2,14 = 23.16, P < 0.001).
