Figure 5

LGR5 expression in secretory cell outgrowth (SCOUT), serous tubal intraepithelial carcinoma (STIC) and high-grade serous carcinoma (HGSC). Combined in situ hybridization for LGR5 and immunohistochemistry for PAX8 and FOXJ1 shows increased LGR5 expression in the SCOUT (A) and STIC (B) lesions in which PAX8-positive cells proliferate. (C) Representative images of LGR5 expression in HGSC. LGR5 expression is frequently observed not only in carcinoma cells but also in adjacent stromal cells. Scale bar, 0.5 mm (upper panels) and 50 µm (lower panels). (D) In some cases, LGR5 expression is only detected in stromal cells. Scale bar, 0.5 mm (upper panels) and 50 µm (lower panels). (E) A bar graph showing the histo-scores (H-scores) of LGR5 in in the nontumor fallopian tubes (NFTs) adjacent to STIC lesions (n = 21), STIC lesions (n = 21), and HGSC lesions (n = 64). The data are shown as the means ± SD. ns, not significant. *P < 0.05 by Tukey’s multiple comparisons test.